Journal: Cell Death & Disease

Article Title: Disruption of the FasL/Fas axis protects against inflammation-derived tumorigenesis in chronic liver disease

doi: 10.1038/s41419-019-1391-x

Figure Lengend Snippet: a Representative TUNEL staining of 8-week-old NEMO f/f , NEMO Δhepa , Fas lpr , and NEMO Δhepa /Fas lpr livers. b TUNEL-positive cells were quantified and graphed. c Proliferation was determination by Ki-67-positive cells immunofluorescence. d Ki-67-positive cells were quantified and graphed. e Expression of PCNA, cleaved Caspase-3 (CC3), and RIPK1 was analysed by immunoblotting of whole liver extracts. GAPDH served as a loading control. Results are expressed as mean ± SEM ( n = 8 livers, p < 0.001–0.05)

Article Snippet: Isolated protein samples were probed with antibodies against RIPK1 (#5389) (Pro-Sci, Poway, CA, USA), Cleaved Caspase-3 (Asp175) (Cell Signaling Technology, Massachusetts, USA), PCNA (clone PC10) (Dianova GmbH, Hamburg, Germany), and GAPDH (MCA4739; 1:5000; AbD SeroTec, Düsseldorf, Germany).

Techniques: TUNEL Assay, Staining, Immunofluorescence, Expressing, Western Blot

Journal:

Article Title: T-cell factor 4 (Tcf7l2) maintains proliferative compartments in zebrafish intestine

doi: 10.1038/sj.embor.7401071

Figure Lengend Snippet: The absence of cycling cells in tcf4exI/exI at 6 weeks post-fertilization. (A) Haematoxylin and eosin (HE) staining of tcf4wt/wt zebrafish intestine with the proximal (Prox), middle (Mid) and distal (Dis) parts magnified respectively in lower panels. (B) PCNA staining on consecutive paraffin sections of wild-type (wt) fish. The arrows in the magnified lower panels indicate PCNA+ cells in the interfold pockets. (C) HE staining of tcf4exI/exI intestine. (D) PCNA staining on consecutive sections depicting absent proliferation in the middle and distal intestinal portions. Note that proliferation in the proximal intestine is maintained (arrows and arrowheads in the magnified proximal intestine in (D)). PCNA, proliferating cell nuclear antigen; wpf, weeks post-fertilization. Scale bars: top panels (A–D), 250 μm; middle and bottom panels (A–D), 50 μm.

Article Snippet: IHC staining with antibody for PCNA (PC10; Euro Diagnostica, Arnhem, The Netherlands) and BrdU (Becton Dickinson, Franklin Lakes, NJ, USA) was carried out as described previously ( van de Wetering et al , 2002 ).

Techniques: Staining

Journal:

Article Title: T-cell factor 4 (Tcf7l2) maintains proliferative compartments in zebrafish intestine

doi: 10.1038/sj.embor.7401071

Figure Lengend Snippet: Gut phenotype of homozygous Tcf4 mutant ‘escaper' fish. (A) Escaper compared with sibling is reduced in size. (B) Western blot analysis of Tcf4 protein from brain and fin tissues showing the absence of protein in escapers. (C) PCNA IHC in wild type (wt). The lower panel is a magnification of the boxed area; the arrows point to PCNA+ cells at the base of the folds. (D) PCNA IHC on Tcf4 escaper fish showing large areas of intestinal epithelium with loss of proliferation. The three lower panels are magnifications of the boxed areas. The arrows point to severely affected areas or to rare PCNA+ patches unequally distributed throughout the epithelial folds. The dashed line indicates the flat epithelial layer in this fish. IHC, immunohistochemistry; PCNA, proliferating cell nuclear antigen; Tcf-4, T-cell factor.

Article Snippet: IHC staining with antibody for PCNA (PC10; Euro Diagnostica, Arnhem, The Netherlands) and BrdU (Becton Dickinson, Franklin Lakes, NJ, USA) was carried out as described previously ( van de Wetering et al , 2002 ).

Techniques: Mutagenesis, Western Blot, Immunohistochemistry

Journal: Journal of Translational Medicine

Article Title: VEGF111b, a C-terminal splice variant of VEGF-A and induced by mitomycin C, inhibits ovarian cancer growth

doi: 10.1186/s12967-015-0522-0

Figure Lengend Snippet: VEGF111b overexpression inhibited growth of SKOV3 tumor xenografts. After transfection with empty lentivirus and VEGF111b lentivirus, SKOV3 cells were injected subcutaneously into the upper right flank region of nude mice. Tumor volume was calculated every 4 days for 20 days. ( a ) Tumor size of the 20th day tumor incubation was displayed. ( b ) Tumor volume was measured with a caliper rule every 4 days. Datas were presented as the mean tumor volumes of mice in both VEGF111b and empty lentivirus vector groups on the days incubation (*** p < 0.001). ( c ) Average tumor weight was shown at the end of the experiments (*** p < 0.001). ( d ) Tumor sections were stained positive for Ki67 and PCNA. The positive products localized in nucleus (magnification, 200×). ( e ) Tumor sections were stained positive for CD31 and VEGF. The positive products localized in cytoplasm (magnification, 200×). Bar heights represent average of data and bars represent the SEM of data. P value <0.05 was considered statisticall

Article Snippet: PCNA mAb (PC10, 1:100), Ki67 mAb (7B11, 1:100), VEGF pAb (ZA-0580, 1:100) and CD31 mAb (1A10, 1:75) were purchased from ZSGB-BIO (Beijing, China).

Techniques: Over Expression, Transfection, Injection, Incubation, Plasmid Preparation, Staining